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The ratiometric readout of RT only responds to GSH (10 mM), but not to glycine (10 mM), cysteine (100 M) or a series of ROS/RNS (100 M)

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For each pulldown or binding assay, 1 mg of lysate or 1 g of recombinant YBX1HA protein was diluted in the reaction buffer containing protease inhibitor (APExBIO, 1007), RNaseOUT recombinant ribonuclease inhibitor (Invitrogen, 10777019) and 260 l of 2 TENT buffer (20 mM Tris-HCl pH 8.0, 2 mM EDTA, 500 mM NaCl and 1% v/v Triton X-100) and polysome extraction buffer (20 mM Tris-HCl pH 7.5, 100 mM KCl, 5 mM MgCl 2 and 0.5% NP-40) in a final volume of 520 l