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For hair growth, the credible endpoints are well established standardized global photography assessed by blinded evaluators, and target-area hair counts and density measured by trichoscopy or phototrichogram at fixed scalp sites over a sufficient duration (typically 24 weeks or more, because the hair cycle is slow)

There is no single best time of day to take these vitamins

Most researchers switch from one to another, not combine them

Williams, T

If you are not going to use the full reconstituted vial within 28 days, consider whether your protocol requires you to reconstitute the full vial at once, or whether you can keep the lyophilised remainder sealed and reconstitute in smaller portions as needed