doi: 10.1210/en.2007-1743 57 HayesMRLeichnerTMZhaoSLeeGSChowanskyAZimmerDet al
Antibodies The following antibodies were used for western blotting (WB) and/or immunofluorescence staining (IF) at the following dilutions: mouse anti-CIP4 (BD, clone 21, 612556, 1:100 for IF, 1:1000 for WB), mouse anti-GAPDH (Santa Cruz, sc-166574, 1:2000 for WB), rabbit anti-WAVE2 (Cell signaling, clone D2C8, #3659, 1:100 for IF, 1:1000 for WB), rabbit anti-N-WASP (Cell signaling, clone 30D10, #4848, 1:1000 for WB), mouse anti-dynamin II (BD, #610263, 1:1000 for WB), mouse anti-endophilin II (Santa Cruz, #sc-365704, 1:1000 for WB), rabbit anti-clathrin light chain A (CLTA) (proteintech, #10852-1-AP, 1:1000 for WB), mouse anti-Rac1 (Millipore, 05-389, 1:4000 for WB), mouse anti-Rac1 (BD, #610651, 1:200 for WB of EV uptake, 1:400 for ELISA), rabbit anti-Cdc42 (Cell signaling, #2462, 1:1000 for WB), rabbit anti-HaloTag (Promega, #G9281, 1:1000 for WB, 1:1000 for IF, 1:1000 for ELISA), mouse anti-HaloTag (Promega, #G9211, 1:1000 for WB, 1:1000 for ELISA), mouse monoclonal anti-MIM (Abnova, #H00009788-M01, 1:1000 for WB), mouse anti-CD81 (Santa Cruz, #sc-166029, 1:2000 for WB), mouse anti-Annexin I (Santa Cruz, sc-12740, 1:1000 for WB), rabbit anti-EEA1 (Cell signaling, #3288, 1:100 for IF), mouse anti-Lamp1 (Santa Cruz, #sc-20011,1:50 for IF), mouse anti--actin (Santa Cruz, #sc-47776, 1:3000 for WB), mouse anti-mCherry (Novus, #NBP1-96752, 1:1000 for IF), rabbit anti-GFP (MBL Life Science, #598, 1:4000 for WB), and rabbit anti-RFP (MBL Life Science, #PM005, 1:3000 for WB)
These differences often come down to personal motivation and attitudes toward health
They checked in on me regularly to see how I was handling the increased dosage and to adjust things if needed
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