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Given that the intestinal epithelial barrier function was found to be positively regulated by activation of the intestinal cannabinoid type 1 receptor (CB1R) 27 , we treated mice with ACEA (arachidonyl-2-chloroethylamide), a selective CB1R agonist 28 during the autoimmune noninflammatory pre-phase of CIA from day 17 to day 27 after immunization