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10.3389/fphys.2019.01102 107

Enzyme Activities For enzyme activities, cells and mouse tissues (duodenum, liver, spleen) were homogenized with a TissueLyser II (Qiagen, Hilden, Germany) in Tris buffer (100 mM Tris (Carl Roth), 300 mM KCl (Applichem, Darmstadt, Germany), pH 7.6 with 0.1% ( v/v ) Triton X-100 (Serva, Heidelberg, Germany), and 0.1% ( v/v ) protease inhibitor (Merck/Millipore, Burlington, MA, USA)) two times for 60 s at 30 Hz

It is used to treat vitamin B12 deficiency in people with pernicious anemia and other conditions

In Pakistan, the sun is strong most of the year, so SPF is non-negotiable

Following digestion of pC210 with Nde I and Sph I to remove the SSA1 coding sequence, similarly digested gliT PCR product was ligated into pC210 to create pC-GliT