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Epitalon-treated oocytes, however, showed reduced Annexin-V signals and a lower proportion of apoptotic cells, indicating that the peptide reduces apoptotic susceptibility in aged oocytes [2]

For each sample, at least three random fields were captured, and the percentage of EdU-positive cells was calculated by dividing the number of EdU-positive nuclei by the total number of Hoechst-stained nuclei