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Peak appetite reduction occurs around week 12 when reaching maintenance dose

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Thus, it is essential to unravel weight loss-induced changes in the composition of AT to identify the relevant cell types as well as the intracellular mechanisms and intercellular communications that maintain an obesogenic memory in AT

After TEV digestion overnight at 4 C, the elution was then subjected to Strep-Tactin Sepharose resin (IBA, 2-1201-010), washed with wash buffer and eluted with wash buffer supplemented with 10 mM desthiobiotin (Sigma-Aldrich, D1411)