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Despite being a potentially critical antioxidant, not much is known about the acute effects of CS on the GSH adaptive response and how GSH levels can fluctuate both during and between CS exposures especially in the lung ELF

There are a multitude of successful differentiation protocols, mostly based on a combination of cell culture medium with different growth supplements, nicotinamide and beta-mercaptoethanol with an average protocol duration of 17 days (Table 3)