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In vitro embryo culture At 18 h post insemination (hpi), the putative zygotes were washed by repeated pipetting and transferred to 25 L drops (approximately one embryo per L) of culture medium (SOF supplemented with 3 mg/mL bovine serum albumin), covered with mineral oil and cultured until Day 8 post insemination (dpi) at 38.5 C in a humidified atmosphere with 5% CO 2 , 5% O 2 and 90% N 2 in air

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More often than not, this reaction is slowly and silently occurring within us, and doing damage to our bodies before we know the damage is done