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The 0.1 ml of supernatant was added to the medium consisting of 0.8 ml 0.1 M TrisHCl buffer (pH 8.9) with the addition of 6 mM EDTA, 12 mM sodium azide, 0.1 ml 4.8 mM GSH, 1 ml 20 mM Luperox (SigmaAldrich, Saint Louis, MO, USA), 0.1 ml 10 mM DNTB

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