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You cannot eliminate dissolved oxygen entirely, but you can minimize exposure by keeping the vial sealed (minimizing headspace), storing it upright (reducing the air-liquid interface), and using the solution within the 28-day window

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Pregnancy and breastfeeding , as safety has not been fully established

A 10 gallon half land/half water aquarium or woodland terrarium is suitable for one frog, but two or more frogs will require a larger space to reduce stress and competition

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