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The expression levels for each of the genes were normalized to fragments per kilobase of exon model per million mapped reads (FPKM) using a software package called RSEM 79

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TB-500: not FDA-approved

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[14] [15] A proportionate assessment should be conducted, avoiding unnecessary investigations while remaining vigilant for 'red flags' that might suggest alternative diagnoses (such as persistent joint swelling, unexplained weight loss, night pain, fever, or focal neurological symptoms)