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The pH is determined by the relative concentrations of the weak acid and the ions

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Concretely, the accumulated lactate promotes CIRP lactylation and consequently leads to CIRP release in macrophages, which then binds to ZBP1 in pulmonary vascular endothelial cells (PVECs)

Next, the sections were incubated with horseradish peroxidase-conjugated anti-rabbit antibodies (Alpha Diagnostic, San Antonio, TX, United States) for 20 min in a 37C incubator and then rinsed with PBS three times for 5 min each

However, in the case of selenoprotein messenger ribonucleic acid (mRNA), the 3untranslated region contains selenocysteine insertion sequence (SECIS) elements, which allow for the recognition of UGA as a selenium cysteine codon rather than a stop signal (21)