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Psychiatry 14 , 90 (2024)

They were studying the cytoprotective proteins in human gastric juice specifically how the stomach lining protects itself from the very acid it produces

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the lysate was clarified by centrifugation at 15,000 nx g for 15 min, protein concentrations were determined using the BCA total protein assay (Pierce), and then appropriate volumes of the lysates were diluted with 2x reducing Laemmli buffer and heated for ten min at 95 C

The synergistic or antagonistic effects of combined compounds must be thoroughly investigated, often requiring preliminary studies to establish safety and efficacy profiles