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19 LiQGuanXWuPWangXZhouLTongYet al
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Results Cystine deprivation leads to rapid death of TNBC cells by mitochondrial oxidative stress independent of GSH loss To investigate the dependence of BC cells on Cyss, we first examined the cytoplasmic Cys/Cyss content in TNBC (Hs 578T and MDA-MB-231) and non-TNBC cell lines (MCF7), cultured for 8 h in the absence of Cyss or in the presence of buthionine sulfoximine (BSO), an inhibitor of GCL (Fig
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Of further interest, the 7 newly formed interfacial salt bridges constitutes a structural electrostatic scaffold (Figures 6, 7, 8, 9, 10 and 11) at the binding interface of GLP-1R ECD and semaglutide analogue A747, which consists of three sets of interfacial salt bridges, as shown by three red numbers (1, 2 and 3) within black circles in Figures 9, 10 and 11
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