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The cells were collected after treatment, washed twice with cold phosphate-buffered saline, and treated with 1 mL of cold citrate buffer (0.24 M sucrose, 40 mM sodium citrate, pH 7.6)

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1c), and an intracellular helical domain (ICH) that links TM6 of the N-terminal bundle and TM7 of the C-terminal bundle