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In December 2014, it was deemed a suitable option for chronic weight management in addition to a calorie reduced diet and physical activity

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After cloning in pGEMT (Promega) the resulting plasmids (Supplementary Table S1) were opened at the unique Sma I site where we cloned the Km r cassette (a Hin cII fragment of the commercial pUC4K plasmid) in the same orientation as the coding sequence it replaced