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B) Under conditions of APAP overdose along with iron overload, excessive cellular free iron reacts with mitochondrial reactive oxygen species (ROS) to induce lipid peroxidation (LPO) of membranes

And, all the rats were raised at Animal Care and Use Committee of Xiamen University (Ethics number XMULAC20190146)

Monitoring intracellular levels of glutathione and thiols involved in its metabolism is important for the proper characterization of cellular injury

Sample preparation for LC-MS/MS typically involves steps such as protein precipitation or liquid-liquid extraction to isolate the target vitamins from the complex biological matrix before chromatographic separation and subsequent mass spectrometric detection (Konieczna et al., 2016