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You may have a deficiency if you are vegan, if you have other health conditions which mean you are unable to absorb it properly such as pernicious anaemia, you take disruptive medications such as antacids or other lifestyle factors such as alcohol intake
Samples were separated on SDSPAGE gels, transferred to PVDF membranes and blocked in 2% fatty acid-free BSA and 5% skim milk powder in PBS containing 0.2% Tween-20 (PBST) for lipoate detection, or 5% skim milk powder in PBST for glutaryl-lysine detection
Mitochondrial profiling Cells were stained with NAO (ThermoFisher, A1372), tetramethylrhodamine, ethyl ester perchlorate (TMRE
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doi: 10.1021/acs.est.1c00522 6 WoodruffTJSuttonP
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