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The concentration of ethanol solution varied with the modeling time, increasing from 520% (mice were offered with 5% ethanol (v/v) for 1 week, then 10% ethanol (v/v) for the next 2 weeks, and 15% ethanol (v/v) for the next 3 weeks, finally animals had access to 20% ethanol (v/v) for last 4 weeks)

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given known sex differences in metabolic and circadian physiology, whether female mice exhibit similar regulation of CI in adipose tissue is to be determined

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Five identical 0.25 ml cell suspensions were prepared, to which we added TGF-1 (20 g/ml, Cat