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4.4 MitoSOX Red and PI stainings Mitochondrial superoxide was measured with MitoSOX Red fluorescent indicator (Thermo Scientific)

In the writhing test, the dose-response curve and the experimental effective dose 50 (ED50) of Rut in different dose combinations were measured

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[5] [6] Prevalence Ehlers-Danlos syndrome affects both males and females

Anti-mouse or anti-rabbit secondary antibodies (Cell Signaling Technology) HRP-conjugated at 1:5000 dilution were incubated with the membranes for 1 h at room temperature, and visualized using a chemiluminescence system