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Proteins (20 g) were separated on 12% SDSpolyacrylamide gel electrophoresis (PAGE) gels, transferred to nitrocellulose/polyvinylidene fluoride membranes, blocked (5% milk in Tris-buffered saline with Tween 20 for 1 h at room temperature (r.t.)), and incubated overnight at 4 C with the following primary antibodies: FSP1 (1:10, clone 6D8-11, Helmholtz Munich), GPX4 (1:1,000, Abcam cat

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Mechanistic studies need to be performed to understand what drives GLP-1R upregulation and the increase in pro-tumorigenic transcription