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N -acetyl-l-Asp, N -acetyl-l-Phe, N -acetyl-l-Tyr, N -acetyl-l-His, and N -Acetyl-l-Lys are scarcely hydrolyzed

High-dose intraperitoneal N-acetylcysteine (800 mg/kg) induced acute liver, kidney, and spleen damage in mice, leading to mortality

Administering hepsin to wild-type mice increases APAP tolerance and downregulates GJ expression Considering the potential clinical and medical applications, we evaluated a therapeutic strategy involving the administration of hepsin to wild-type adult mice and assessed its potential to mitigate APAP-induced liver injury

The exact amount of proteins was resolved on 420% SDSPAGE gel for separation and was electroblotted onto 0.22-m PVDF membranes (Merck-Millipore, CA, USA)