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Denaturation and reduction steps were optimized to facilitate enzymatic digestion without compromising the lipidated moiety. Enzymatic Digestion A dual-enzyme approach employing Glu-C and chymotrypsin was utilized to generate overlapping peptide fragments, ensuring full sequence coverage

Other Uses Glutathione is an antioxidant that may be beneficial for general wellness support

Important Notes Sample concentrations should be estimated before being used in the assay and a proper dilution factor should be selected to make the diluted target protein concentration fall in the optimal detection range of this kit

91 ShenZ.HuangD.JiaN.ZhaoS.PeiC.WangY.et al (2023)