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Nuclear magnetic resonance spectroscopy 420 l of culture medium, 120 l of 1 mM 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS) in deuterium oxide (D 2 O) as an internal standard, and 60 l of 500 mM phosphate buffer, pH 7 were mixed and transferred to 5 mm glass NMR tubes for analysis

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