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The microsomes were incubated with 500 M solutions of Dihexa, piroxicam, verapamil, and 7-ethoxycoumarin, as controls for low, moderate, and highly metabolized compounds, respectively, in 0.1 M Tris buffer with an NADPH-regenerating system at 37C

This confirms the anti-apoptotic effect through a second, independent measurement

Relationship to previous work Cell clusters are common in degenerated discs, especially those that are herniated 36 , and are associated with cell proliferation 12,37 and with progenitor cells 38

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