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Cultures were then diluted 1:500 to 1:1,000 in TSB or Seahorse XF DMEM (Agilent) supplemented with 100 mM glucose, 2 mM L-glutamine and 1 mM sodium pyruvate, and dispensed into poly-D-lysine (PDL)-coated Seahorse XF HS miniplates (100 l per well)

Higher red/green ratios indicate healthier mitochondrial activity, which is crucial for oocyte competence and embryo development [2]

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However, these studies all examined one-time switches, not repeated cycling

Id long thought of lifting weights as essential for athletic performance, regardless of your sport, but I hadnt considered the impact on healthy aging