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The lysate was cleared by centrifugation and the protein was purified by Ni-His-affinity chromatography in batch mode

Individuals with genetic variants associated with reduced GST activity or impaired antioxidant enzyme function may require higher glutathione support or more frequent dosing schedules

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These results established EV-derived VDAC1 protein as a novel therapeutic target for chemoresistant GC and identify DIDS as a potential agent for reversing PTX resistance

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