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The indicated plasmid combinations were used for the protein-protein interaction test according to the manufacturers instructions (Clontech, Phenotype characterization In vitro pollen germination was conducted in a Petri dish containing solid PGM [1% low melting point agarose (w/v), 18% sucrose (w/v), 0.01% boric acid (w/v), 1 mM CaCl 2 , 1 mM Ca(NO 3 ) 2 , and 1 mM MgSO 4 , adjusted to pH 7.5 using 0.2 M KOH] at 28 C, following a protocol modified from Boavida and McCormick (2007) 67

However, when H 2 O 2 is used, GPx1 plays a major role in reducing H 2 O 2 to block subsequent downstream events such as lipid peroxidation [19]

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The flavor is pretty good, and the packets are so convenient

Engineering 3 (1), 115121