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Cell viability assay For cell viability assay, cells were planted in 96-well plates (34 10 3 per well) after indicated treatment, then incubated with 10% CCK-8 reagent at 37 C for 2 h

All reconstitution and transfer processes should occur within a sterile environment, ideally under a laminar flow hood, to prevent contamination

Your goal is to create a solution with a known concentration

Color Change: While GHK-Cu solutions are typically a clear to light blue hue (due to the copper), any significant deviation, such as turning dark blue or green, or developing a yellowish tint, could indicate degradation

Benade V, Jayarajan P, Krishnadas N, Kamuju V, Gandipudi S, Nirogi R (2021) Metformin, a calorie restriction mimetic for the treatment of Alzheimers disease: a preclinical investigation