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Osman NI, Bratt DG, Downey AP, Esperto F, Inman RD, Chapple CR

mTORC1 activation on membranes We used large unilamellar vesicles (LUVs), with a lipid composition of 72.8% DOPC, 7% POPS, 10% cholesterol, 5% DGS-nitrilotriacetic acid (NTA), 5% PE MCC and 0.2% DiR, as the membrane platform to investigate the role of RAGRagulator and RHEB in mTORC1 activation

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It's a fundamental step that, if mishandled, can compromise an entire experiment, leading to wasted time, resources, and potentially misleading data