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The active site of GPX4, selenocysteine, alternates between reduced and oxidized states to sustain its catalytic activity

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Of which est1 , the maximally induced esterase, does not belong to any of the known families of esterases, while the next esterase in the descending order of induction profile is est2 , affiliated to Group VIII, comprised of -lactamases and had been described as di-monoesterase, which is supposed to metabolize both phthalate diester as well as phthalate monoester, followed by est3 , which belongs to Group M and comprises of phthalate monoesterase only