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Although AFLD is usually considered a benign and static lesion, it can develop irreversible liver damage in a short period of time, MASLD is caused by high-fat diet (HFD), high-fructose diet, methionine- and choline-deficient diet (MCD), gene mutations, and heavy-metal poisoning

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The concentration of each analyte in the samples was determined from calibration curves by using the peak area ratio of the analyte to its corresponding isotope internal standard

I cannot advise but I am sure the right person will come alomg with the right explanation and not all that keep it sterile stuff which is all well and good but nothing is sterile once it is opened

Model robustness was assessed using Receiver Operating Characteristic - Area Under Curve (ROC-AUC) analysis in MetaboAnalyst software