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NCs may maintain the young NP phenotype by secreting factors that suppress cell death and by influencing genes that regulate IVD anabolic activity and matrix protection [66]

The medium was then replaced with fresh medium containing DZN (0300 M) and further cultured for 24 h

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[8] Once activated, these G-proteins may stimulate the production of second messengers like cAMP or IP3, which appear to act as secondary signaling molecules, potentially further propagating the signal within the cell