For immunofluorescent staining, after incubated with primary antibodies, the sections were incubated with fluorescent secondary antibody Goat anti-Mouse IgG 488 (A11001, Thermo Scientific), Goat anti-Mouse IgG 594 (A11005, Thermo Scientific), Goat anti-Rabbit IgG 488 (A11008, Thermo Scientific), Goat anti-Rabbit IgG 594 (A11012, Thermo Scientific) for 1h at room temperature
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Since the band broke in 2015 our fans in India have been so passionate and vocal about loving the music and wanting us to visit, says Gonzalez
The hippocampus helps bind those elements into a memory that can later be retrieved
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