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When the cigarette is smoked, the nicotine concentrates down in the oral end meaning that a small nub of a cigarette butt will retain 25% of the nicotine contained in the original cigarette

Other important differences between e-cigarette aerosols and combustible tobacco cigarette smoke in such systems include aerosols condensing in transit tubing (possibly restricting aerosol flow and impeding syringe function) and some concerns with device button activation synchrony (either manually, or automated with a separate robot) with the syringe puffing to ensure the entire puff is activated and delivered (Goniewicz et al., 2014

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Use a clean cut that opens the cap without taking too much off

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