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After the antibodies bind to a specific antigen in the tissue sample, the enzyme or dye is activated, and the antigen can then be seen under a microscope

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(165,000 deaths) of these deaths were from lower respiratory tract infections.Cut off point of cotinine level in the serum of children exposed to second hand smoke was 2.24 ng/ml with high sensitivity and specificity (87.5%, 100% respectively) which signify that it is sensitive and specific marker in evaluation of SHS metabolites in second hand smoke children