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The tissue inserts were transferred into 1 mL of fresh culture medium, which bathes the basolateral side of the tissue, and exposed to the apical surface to DA vapor for 1 h at 37 C (+5% CO 2 ) using vapor cups (MatTek)

In the case of smoke, this affects, among other things, part of the aromatic and sulphur-containing gases that are partly responsible for a scratchy taste and pungent smell

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The goal is to keep the benefits of breast milk while lowering the risks from smoking

The initial expense is, once again, higher but with better battery life and more ability to customize components, the overall costs end up being much lower