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This is achieved through blending different types of tobacco, ventilation, addition of accelerants, and filtration, as discussed in Chapter 4

Other health hazards Eyes Chemicals in tobacco cause damage to the macula

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Real time quantitative PCR analysis Real-time quantitative PCR amplification was conducted by pipetting 4.4 L of cDNA (diluted 1:25 or 1:50 in MilliQ water), 5 L 2xSensiMix SYBR and Fluorescein Kit (Bioline, the Netherlands) and 0.6 L forward and reverse primers for target genes in white LightCycler480 384 multiwell plates (Roche)

Zhou K, Zhao R, Geng Z, Jiang L, Cao Y, Xu D, Liu Y, Huang L, Zhou J