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It is a complex brain disease characterized by compulsive, at times uncontrollable drug craving, seeking, and use despite devastating consequences - behaviors that stem from drug-induced changes in the brain." Scientists have documented how nicotine physically alters the brain

Confocal microscopy to measure airway epithelial cell layer integrity To determine the effect of CSE on epithelial intercellular adhesion, coverslips were coated with poly-L-lysine solution (Sigma, MO, USA) for 5 min at RT, washed with PBS, and dried for 1 h at 60 C

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