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2.5 Determination of nitrogenous compound content 2.5.1 Determination of total alkaloid content The determination of total alkaloid content follows the method outlined inTobacco and tobacco productsDetermination of total alkaloidsContinuous flow (potassium thiocyanate) method:YC/T468-2021 ( The total alkaloid content (expressed as nicotine) on a dry basis (a), in percentage (%), was calculated using the following formula: CInstrumental observation value of total alkaloids in the extraction solution (mg/mL), vVolume of the extraction solution (mL), mMass of the sample (mg), wMass fraction of moisture in the sample