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After the medium had solidified, BCEV-treated SV-HUC cells were suspended at 1 10 5 cells/mL in medium containing 0.4% Noble agar and added atop the established 0.8% agarose layer (6 wells per treatment condition)

Our main aim, though, is for animal use to be phased out entirely and replaced with humane alternatives

They can be accompanied by convulsions, where the body shakes uncontrollably