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Data analysis Participant demographics and smoking history were assessed with descriptive analyses and were compared across study sites using chi-squared test and one-way ANOVA

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An occlusal splint was made for the patient

Materials and methods Cell lines and drug treatment The human bronchial epithelial cell line (16-HBE) was obtained from a cell bank (American Type Culture Collection [ATCC], Manassas, VA, USA) and cultured in Dulbeccos Modified Eagles Medium (DMEM