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Novel high-throughput single-cell approaches, including RNA 171 and bisulphite sequencing 172 , as well as assay for transposase-accessible chromatin (ATAC) 173 and Cleavage Under Targets and Tagmentation (CUT&Tag) 174 allow transcriptomic and epigenomic profiling of low-input heterogeneous tissue at single-cell or -nucleus resolution

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Despite the warped logic of taking out the relatively benign nicotine and leaving the very harmful tar, despite the obvious practical problems of mounting a de facto cigarette prohibition at this scale, and despite the weird ethics of encouraging people to switch from one smoking product to another smoking product, this idea just will not die