To create a differentiated airway epithelial cell culture grown on ALI, basal cells were isolated using 0.05% trypsin (in 0.7 mM ethylenediaminetetraacetic acid EDTA (Gibco, ThermoFisher Scientific, Waltham, MA) and seeded at a density of 10 5 basal cells on collagen IV-coated Costar Transwell inserts (6.5 mm, 0.4 m
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We thus here sketch a roadmap for application of multiscale in silico models, suggesting how the power of mathematical and computational modeling methods may augment and illuminate this significant public health problem