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For phospho-protein staining, cells were treated with the indicated smoking-related stimuli for an appropriate amount of time, after which they were resuspended in 100 L of culture medium and fixed with an equal volume of BD Cytofix fixation buffer for 15 min at 37 C, resuspended in 150 L of BD Cytoperm Solution III for 30 min on ice, and resuspended in PBS containing 2% FBS and PE Mouse Anti-H2AX (pS139) (1:50) or PE Mouse anti-NF-B p65 (pS529) (1:50) for 20 min at room temperature

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Mod : short for modification, this refers to the way in which an e-cig has been modified or adapted to suit a particular users tastes