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MSCs exposed to both cigarette and e-cigarette smoke extracts generated high levels of ROS, as defined by increased superoxide radical levels 54,63,64 , which can explain attenuated differentiation of smoke-exposed cells 65

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Both of these shifts may be due to changes in cigarette design and composition, in how tobacco leaves are cured, and in how deeply smokers inhale cigarette smoke and the toxicants it contains (1, 8)