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Sensitivity analysis showed that the global AAPC calculated under different time windows showed a decreasing trend in the same direction, despite minor differences in the estimates

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In contrast, the distance between inter-group samples was large, indicating significant differences between the two groups (Figure 2A)

Samples were visualized on a Leica SP8 confocal laser scanning microscope (Leica Microsystems, Wetzlar, Germany) with a 63 oil immersion objective, a 488 nm diode laser and 520 nm Alexa Fluor 488 detection filter (green antibody signal) and a 405 nm diode laser in combination with a Hoechst 33342 detection filter (blue nucleic signal)