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The Alkaline Comet Assay in Peripheral Blood Lymphocytes The alkaline version of the comet assay was used to evaluate DNA damage in PBLs from each subject and was carried out as described elsewhere ( ex vivo challenge, 20 L of whole blood was added to 1 ml PBS (Gibco-Invitrogen) and exposed to 32 mM of ethyl methanesulfonate (EMS, Sigma), incubated at 37C for 30 min, and equally processed