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After the addition of 100 L of internal standard (d 4 -nicotine in acetonitrile), 400 L ammonia (5 %) and 10 mL acetonitrile, the mixture was shaken thoroughly (15 min) and ultra-sonified (15 min)

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Shelley Long had just left Cheers , the wildly popular, influential NBC sitcom that was built entirely on the scorching, hilarious romantic chemistry between Longs pretentious Diane Chambers and Ted Dansons cocky Sam Malone